Journal: Bioengineering

Article Title: Formation and Long-Term Culture of hiPSC-Derived Sensory Nerve Organoids Using Microfluidic Devices

doi: 10.3390/bioengineering11080794

Figure Lengend Snippet: Characterization of neurospheres and axon bundles using fluorescent immunostaining. ( a , b ) TUJ1 as a neuron marker and ( a ) ISL1 as a sensory neuron marker. An organoid cultured for 39 days was used. ( b ) TRPV1 as a nociceptor marker. An organoid cultured for 28 days was used. White-colored scale bars: 500 μm. Gray-colored scale bars in close-up panels for axon bundles: 100 μm.

Article Snippet: For molecular characterization, the sensory nerve organoids were labeled with anti-tubulin β3 (TUBB3/TUJ1) mouse monoclonal antibody (MMS-435P, BioLegend, CA, USA), anti-ISL LIM homeobox 1 (ISL1) rabbit polyclonal antibodies (GTX102807, GeneTex, TX, USA), or anti-TRPV1 rabbit polyclonal antibodies (NBP1-97417, Novus Biologicals, CO, USA), followed by incubation with Alexa Fluor-conjugated anti-mouse or anti-rabbit polyclonal secondary antibodies (ab150109, ab150068, Abcam, Waltham, MA, USA).

Techniques: Immunostaining, Marker, Cell Culture

Journal: Frontiers in bioscience (Landmark edition)

Article Title: Single-Cell Transcriptome Analysis of Small Cell Neuroendocrine Carcinoma of the Endometrium Reveals ISL1 as a Potential Biomarker for Diagnosis and Treatment.

doi: 10.31083/j.fbl2903100

Figure Lengend Snippet: Fig. 5. ISL1 modulates neuroactive pathway in subclusters of single cell RNA-seq data and The Cancer Genome Atlas (TCGA)

Article Snippet: After cell counting, the cells were incubated with ISL LIM Homeobox 1 (ISL1) monoclonal antibody (1:200, 15661-1-AP; Proteintech, Rosemont, IL, USA) at 4 °C in the dark for 60 min, followed by incubation for 30 min with a fluorescent secondary antibody (1:200, SA00013-2, CoraLite488-conjugated goat anti-rabbit IgG (H+L)).

Techniques: RNA Sequencing

Journal: Frontiers in bioscience (Landmark edition)

Article Title: Single-Cell Transcriptome Analysis of Small Cell Neuroendocrine Carcinoma of the Endometrium Reveals ISL1 as a Potential Biomarker for Diagnosis and Treatment.

doi: 10.31083/j.fbl2903100

Figure Lengend Snippet: Fig. 6. Flow cytometric sorting of neuroendocrine tumor cells to obtain ISL1 expression group, and explore the differences in cell function in vitro. (A) Flow cytometric sorting of neuroendocrine tumor cell line H446 according to the difference in the expression of

Article Snippet: After cell counting, the cells were incubated with ISL LIM Homeobox 1 (ISL1) monoclonal antibody (1:200, 15661-1-AP; Proteintech, Rosemont, IL, USA) at 4 °C in the dark for 60 min, followed by incubation for 30 min with a fluorescent secondary antibody (1:200, SA00013-2, CoraLite488-conjugated goat anti-rabbit IgG (H+L)).

Techniques: Expressing, Cell Function Assay, In Vitro